TRIM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20356-1-AP

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

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Validation Data Gallery

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  WB analysis using 20356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 20356-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20356-1-AP

  IP result of anti-TRIM2 (IP:20356-1-AP, 4ug; Detection:20356-1-AP 1:300) with mouse brain tissue lysate 3200ug.

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  IHC staining of human kidney using 20356-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20356-1-AP (TRIM2 Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of HeLa using 20356-1-AP

  Immunofluorescent analysis of Hela cells, using TRIM2 antibody 20356-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse small intestine tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 20356-1-AP is TRIM2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM2 fusion protein Ag14208
• Full Name: tripartite motif-containing 2
• Calculated Molecular Weight: 744 aa, 82 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC011052
• Gene Symbol: TRIM2
• Gene ID (NCBI): 23321
• RRID: AB_10734319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

Publications

Species	Application	Title
human	IP,WB,IF	Nat Commun; Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity; Authors - Diego Esposito
human	WB,IF	J Transl Med; Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.; Authors - Zili Zhou
human	WB	Cancer Cell Int; TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.; Authors - Zhaoxian Lin ; KD Validated