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  • KD/KO Validated

TRIM22 Polyclonal antibody

TRIM22 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13744-1-AP

Synonyms

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22



Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13744-1-AP targets TRIM22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM22 fusion protein Ag4683
Full Name tripartite motif-containing 22
Calculated molecular weight 498 aa, 57 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC035582
Gene symbol TRIM22
Gene ID (NCBI) 10346
RRIDAB_2877974
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TRIM22 antibody 13744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.

Authors - Alice Mac Kain
humanWB,IHC

Oncotarget

TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.

Authors - Li Liu
  • KD Validated
humanWB,IHC,IF

Front Cell Infect Microbiol

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Authors - Matthew Charman
  • KD Validated
humanIHC

Immunobiology

Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations

Authors - Tiantian Cai
humanWB

Cell Biol Int

TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

Authors - Liyin Li
  • KD Validated
humanWB

Transl Cancer Res

FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.

Authors - Yang Wang
  • KD Validated