TRIM23 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM23 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12607-1-AP

Synonyms

ARD1, ARFD1, GTP binding protein ARD 1, RING finger protein 46, RNF46, TRIM23, tripartite motif containing 23

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 12607-1-AP

  WB result of TRIM23 antibody (12607-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM23 transfected HEK-293 cells.

• 

  WB analysis of mouse brain using 12607-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12607-1-AP (TRIM23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12607-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12607-1-AP (TRIM23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 12607-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12607-1-AP (TRIM23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12607-1-AP targets TRIM23 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM23 fusion protein Ag3302
• Full Name: tripartite motif-containing 23
• Calculated Molecular Weight: 574 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC022510
• Gene Symbol: TRIM23
• Gene ID (NCBI): 373
• RRID: AB_2256643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.