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  • KD/KO Validated

TRIM25 Polyclonal antibody

TRIM25 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12573-1-AP


EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12573-1-AP targets TRIM25 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM25 fusion protein Ag3241
Full Name tripartite motif-containing 25
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 68-71 kDa
GenBank Accession NumberBC016924
Gene Symbol TRIM25
Gene ID (NCBI) 7706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).


Product Specific Protocols
WB protocol for TRIM25 antibody 12573-1-APDownload protocol
IHC protocol for TRIM25 antibody 12573-1-APDownload protocol
IF protocol for TRIM25 antibody 12573-1-APDownload protocol
IP protocol for TRIM25 antibody 12573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Signal Transduct Target Ther

Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis

Authors - Yuanliang Yan

Nat Immunol

USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation

Authors -
  • KO Validated

Nat Immunol

Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.

Authors - Wang Penghua P

Cell Rep

p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP

Authors - Yingdan Huang


Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.

Authors - Wei Liu


Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.

Authors - Jia Tong


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Iram (Verified Customer) (01-15-2021)

TRIM25 antibody giving a very clear and sharp expression.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer cells and tissue