Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12573-1-AP targets TRIM25 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3241 Product name: Recombinant human TRIM25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 298-630 aa of BC016924 Sequence: SLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK Predict reactive species |
| Full Name | tripartite motif-containing 25 |
| Calculated Molecular Weight | 630 aa, 71 kDa |
| Observed Molecular Weight | 68-71 kDa |
| GenBank Accession Number | BC016924 |
| Gene Symbol | TRIM25 |
| Gene ID (NCBI) | 7706 |
| RRID | AB_2209732 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14258 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM25 antibody 12573-1-AP | Download protocol |
| IHC protocol for TRIM25 antibody 12573-1-AP | Download protocol |
| IP protocol for TRIM25 antibody 12573-1-AP | Download protocol |
| WB protocol for TRIM25 antibody 12573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM25 antibody (Cat# 12573-1-AP) has 49 total citations across journals including Nature Communications, Molecular Cell, Cell Death & Disease, Advanced Science, Cell Reports, eLife, Autophagy, Molecular Cancer, and J Biol Chem. Research fields span cancer biology (glioblastoma, lung, colorectal, breast, gastric, liver, bladder, and endometrial cancers), antiviral innate immunity (influenza, RSV, HIV), autophagy, ubiquitination/protein degradation, ferroptosis, inflammation, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis | ||
Mol Cancer Circ6834 suppresses non-small cell lung cancer progression by destabilizing ANHAK and regulating miR-873-5p/TXNIP axis | ||
Autophagy Bunyavirus SFTSV NSs utilizes autophagy to escape the antiviral innate immune response | ||
Nat Commun Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1 | ||
Nat Commun DSS1 inhibits autophagy to activate epithelial-mesenchymal transition in a pro-metastatic niche of renal cell carcinoma.
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Reviews
What customers say
All three reviewers gave 5-star ratings. Users report excellent performance across Western blot, IHC, IF, and IP applications, with clear and sharp signal expression. One reviewer specifically noted appreciable brightness in IF and IHC. The antibody was used at 1:1000 dilution for Western blot and 1:100 for IHC on human colon cancer cells and tissue. Overall, customers describe it as a reliable, high-quality TRIM25 antibody with consistent results across multiple techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (03-24-2026) | Very good antibody, nice for Western blots!
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Mounika (Verified Customer) (01-08-2026) | Antibody worked very good for Western blot and IF,IHC brightness was appreciating!
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Iram (Verified Customer) (01-15-2021) | TRIM25 antibody giving a very clear and sharp expression.
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