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TRIM25 Polyclonal antibody

TRIM25 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12573-1-AP


EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse pancreas tissue, K-562 cells, MCF-7 cells, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12573-1-AP targets TRIM25 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM25 fusion protein Ag3241
Full Name tripartite motif-containing 25
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight 68-71 kDa
GenBank accession numberBC016924
Gene symbol TRIM25
Gene ID (NCBI) 7706
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790).


Product Specific Protocols
WB protocol for TRIM25 antibody 12573-1-APDownload protocol
IHC protocol for TRIM25 antibody 12573-1-APDownload protocol
IF protocol for TRIM25 antibody 12573-1-APDownload protocol
IP protocol for TRIM25 antibody 12573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Immunol

Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.

Authors - Wang Penghua P


Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.

Authors - Wei Liu


Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.

Authors - Jia Tong


ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour

J Virol

Robust Lys63-linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection.

Authors - Jingwen Jiang

Life Sci

Knockdown of ZBTB7A inhibits cell proliferation of breast cancer through regulating the ubiquitination of estrogen receptor alpha.

Authors - Xiao Xiao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Iram (Verified Customer) (01-15-2021)

TRIM25 antibody giving a very clear and sharp expression.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer cells and tissue