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TRIM25 Polyclonal antibody
TRIM25 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 12573-1-AP
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse pancreas tissue, K-562 cells, MCF-7 cells, rat liver tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 14 publications below|
|IF||See 1 publications below|
|IP||See 1 publications below|
|CoIP||See 2 publications below|
12573-1-AP targets TRIM25 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRIM25 fusion protein Ag3241|
|Full Name||tripartite motif-containing 25|
|Calculated molecular weight||630 aa, 71 kDa|
|Observed molecular weight||68-71 kDa|
|GenBank accession number||BC016924|
|Gene ID (NCBI)||7706|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790).
|Product Specific Protocols|
|WB protocol for TRIM25 antibody 12573-1-AP||Download protocol|
|IHC protocol for TRIM25 antibody 12573-1-AP||Download protocol|
|IF protocol for TRIM25 antibody 12573-1-AP||Download protocol|
|IP protocol for TRIM25 antibody 12573-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.
Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.
Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.
ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.
Robust Lys63-linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection.
Knockdown of ZBTB7A inhibits cell proliferation of breast cancer through regulating the ubiquitination of estrogen receptor alpha.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (01-15-2021)
TRIM25 antibody giving a very clear and sharp expression.