Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, LNCaP cells, COLO 320 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67314-1-Ig targets TRIM25 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29325 Product name: Recombinant human TRIM25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 298-630 aa of BC016924 Sequence: SLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK Predict reactive species |
| Full Name | tripartite motif-containing 25 |
| Calculated Molecular Weight | 630 aa, 71 kDa |
| Observed Molecular Weight | 68-71 kDa |
| GenBank Accession Number | BC016924 |
| Gene Symbol | TRIM25 |
| Gene ID (NCBI) | 7706 |
| RRID | AB_2882574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14258 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM25 antibody 67314-1-Ig | Download protocol |
| IHC protocol for TRIM25 antibody 67314-1-Ig | Download protocol |
| WB protocol for TRIM25 antibody 67314-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TRIM25 antibody (Cat# 67314-1-Ig) has 15 total citations. Citations appear in journals including Signal Transduction and Targeted Therapy, Cell Research, Theranostics, Advanced Science, Cell Death & Disease, Oncogene, Cell Reports, Drug Resistance Updates, Phytomedicine, Respiratory Research, Journal of Cellular and Molecular Medicine, Experimental Biology and Medicine, and eLife. Research fields span glioblastoma, breast cancer, lung cancer, colorectal cancer, pancreatic cancer, cholangiocarcinoma, aortic valve calcification, MAFLD, ferroptosis, and antiviral immunity.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis | ||
Cell Res Destruction of VISTA by TRIM25 ablation in T cells potentiates cancer immunotherapy.
| ||
Drug Resist Updat ITPKB suppresses ER calcium release to promote CXCL9 expression and enhance immunotherapy sensitivity in triple-negative breast cancer. | ||
Theranostics Hsa_circ_0058495-mediated IGF2BP2 ubiquitination and m6A modification of MEKK1 promote the progression of PDAC. | ||
Adv Sci (Weinh) Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis | ||
Cell Death Dis Acetylation-dependent USP7-TRIM25 axis drives oncogenic progression in non-small cell lung cancer. |
