TRIM27 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM27 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (5)

Cat no : 12205-1-AP

Synonyms

Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP

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Validation Data Gallery

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  WB analysis using 12205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12205-1-AP

  WB result of TRIM27 antibody (12205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM27 transfected Jurkat cells.

• 

  WB analysis of mouse testis using 12205-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12205-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12205-1-AP

  IP result of anti-TRIM27 (IP:12205-1-AP, 4ug; Detection:12205-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human lung cancer using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue, Raji cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human lung cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• CoIP: See 4 publications below

Product Information

12205-1-AP targets TRIM27 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM27 fusion protein Ag2849
• Full Name: tripartite motif-containing 27
• Calculated Molecular Weight: 513 aa, 58 kDa
• Observed Molecular Weight: 58 kDa, 41 kDa
• GenBank Accession Number: BC013580
• Gene Symbol: TRIM27
• Gene ID (NCBI): 5987
• RRID: AB_2256660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Elife; The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.; Authors - Zane A Gibbs
human	CoIP	Mol Ther Nucleic Acids; TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination.; Authors - Li Xing
human	WB	FASEB J; TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells.; Authors - Yinfang Wang ; KD Validated
human	WB,IF	J Cell Mol Med; NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.; Authors - Bowen Hu
mouse	WB	J Leukoc Biol; Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes.; Authors - Tongjie Wang
human	WB,IF	FEBS J; TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1; Authors - Juncal Garcia‐garcia ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amy (Verified Customer) (03-18-2024)

Clean bands for U2OS cell lysates 25ug.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

FH

Jason (Verified Customer) (08-17-2022)

Third time purchase. I am satisfied. This antibody works very well in human breast cancer cells using WB at dilution 1:1000, but many non-specific IF singling detected.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB: 1:1000

FH

Elisa (Verified Customer) (12-21-2021)

HAP1 were lysates with RIPA buffer. 1mg of protein was loaded per condition. Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with Trim27 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect TRIM27 signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1

TRIM27 Antibody Western Blot validation (1:1000 dilution) in HAP1 (Cat no:12205-1-AP)

FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (30 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-TRIM27 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T

TRIM27 Antibody Western Blot, validation (1:1000 dilution) in HEK 293T (Cat no:12205-1-AP)

FH

Francesca (Verified Customer) (05-16-2019)

Excellent antibody, clean and strong signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS and A549 cells

TRIM27 Antibody Western Blot, validation (1:2000 dilution) in U2OS and A549 cells (Cat no:12205-1-AP)