TRIM27 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM27 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28751-1-AP

Synonyms

Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP

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Validation Data Gallery

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  WB analysis using 28751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28751-1-AP (TRIM27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28751-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 28751-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 28751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH9).

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  IHC staining of mouse testis using 28751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH9).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Raji cells
• Positive IHC detected in: human colon cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

28751-1-AP targets TRIM27 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM27 fusion protein Ag30576
• Full Name: tripartite motif-containing 27
• Calculated Molecular Weight: 513 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC013580
• Gene Symbol: TRIM27
• Gene ID (NCBI): 5987
• RRID: AB_2881207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IF	Lab Invest; TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway.; Authors - Jinxi Liu ; KD Validated