TRIM27 Recombinant monoclonal antibody, PBS Only

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  IF Staining of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84927-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84927-6-RR against Human TRIM27 were performed. The affinity constant is 0.653 nM.

TRIM27 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84927-6-PBS

Clone No.242538H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242538H5, EC:2.3.2.27, RFP, RNF76, TRIM

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Product Information

84927-6-PBS targets TRIM27 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2849

  Product name: Recombinant human TRIM27 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 165-513 aa of BC013580

  Sequence: EQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP

• Full Name: tripartite motif-containing 27
• Calculated Molecular Weight: 513 aa, 58 kDa
• GenBank Accession Number: BC013580
• Gene Symbol: TRIM27
• Gene ID (NCBI): 5987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P14373
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.