TRIM29 Polyclonal antibody

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  WB analysis using 17542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

TRIM29 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17542-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ATDC, TRIM29, tripartite motif containing 29

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, BxPC-3 cells
• Positive IHC detected in: human skin cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

17542-1-AP targets TRIM29 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM29 fusion protein Ag11600
• Full Name: tripartite motif-containing 29
• Calculated Molecular Weight: 588 aa, 66 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC017352
• Gene Symbol: TRIM29
• Gene ID (NCBI): 23650
• RRID: AB_2272412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14134
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein).ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1(PMID:7644499). ATDC gene is highly expressed in samples of primary pancreatic cancer and PanIN lesions compared to normal pancreas and chronic pancreatitis and the highly expressed protein in human pancreatic cancer,enhances cancer cell proliferation and in vivo tumorigenicity by upregulation of the Wnt/b-Catenin signaling pathway(Wang, L. etc.2007).

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
human	WB	Oncogenesis; Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.; Authors - Chuan-Xia Zhang
human	WB,IHC	PLoS One; Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway.; Authors - Tang Zhong-Ping ZP
		Cancer Sci; Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas.; Authors - Shotaro Yamano
human	WB	Cancer Biol Ther; iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma.; Authors - Kun Wang
human	WB,IHC	Cancer Res Treat; TRIM29 Overexpression Promotes Proliferation and Survival of Bladder Cancer Cells Through NF-κB Signaling.; Authors - Shu-Tao Tan ; KD Validated