Multi-rAb® TRIM29 Multi-Recombinant antibody

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• Multi-rAb® TRIM29 Multi-Recombinant antibody (RMX00086)

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  WB analysis using RMX00086

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00086 (TRIM29 antibody) at dilution of 1:17000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using RMX00086

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using RMX00086 (TRIM29 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using RMX00086

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using RMX00086 (TRIM29 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using RMX00086

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TRIM29 antibody (RMX00086) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TRIM29 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00086

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ataxia telangiectasia group D-associated protein, ATDC, tripartite motif containing 29, Tripartite motif-containing protein 29

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Tested Applications

• Positive WB detected in: A431 cells, HT-29 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00086 targets TRIM29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: tripartite motif-containing 29
• Calculated Molecular Weight: 588 aa, 66 kDa
• Observed Molecular Weight: 65~70 kDa
• GenBank Accession Number: BC017352
• Gene Symbol: TRIM29
• Gene ID (NCBI): 23650
• RRID: AB_3750135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q14134
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein). ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1 (PMID:7644499). Knockdown of TRIM29 in airway epithelial cells enhances type I interferon production, and in human nasopharyngeal carcinoma cells results in almost complete Epstein-Barr virus clearance. TRIM29 is also highly induced by cytosolic double-stranded DNA in myeloid dendritic cells (PMID: 29038422). Western detected both full-length TRIM29 (65 kDa) and a truncated variant of TRIM29 with a molecular weight of about 50 kDa (PMID: 26095369).