TRIM31 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM31 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12543-1-AP

Synonyms

C6orf13, HCG1, HCGI, RNF, TRIM31, tripartite motif containing 31

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Validation Data Gallery

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  WB analysis of COLO 320 using 12543-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12543-1-AP (TRIM31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 12543-1-AP

  IP result of anti-TRIM31 (IP:12543-1-AP, 4ug; Detection:12543-1-AP 1:2000) with HT-29 cells lysate 1040 ug.

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  IHC staining of human small intestine using 12543-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 12543-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IP detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

12543-1-AP targets TRIM31 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM31 fusion protein Ag3264
• Full Name: tripartite motif-containing 31
• Calculated Molecular Weight: 425 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC017017
• Gene Symbol: TRIM31
• Gene ID (NCBI): 11074
• RRID: AB_10640897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM31(Tripartite motif-containing protein 31) is also named as C6orf13, RNF and belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING domain, a B-box-2 domain, and a coiled-coil region. TRIM31 forms homodimers(PMID:11331580). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity.; Authors - Junji Zhu ; KO Validated
human	WB	Nat Commun; USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS.; Authors - Jinxiu Hou
human	WB	Nat Commun; TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells.; Authors - Eun A Ra
mouse,rat	WB,IF	Redox Biol; The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.; Authors - Shenglan Zeng ; KD Validated
human	WB	J Virol; TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.; Authors - Uchil Pradeep D PD
mouse	WB	J Immunol; AKT Regulates NLRP3 Inflammasome Activation by Phosphorylating NLRP3 Serine 5.; Authors - Wei Zhao