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TRIM35 Polyclonal antibody

TRIM35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19806-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of A431 using 19806-1-AP
WB result of TRIM35 antibody (19806-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM35 transfected A431 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 19806-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19806-1-AP (TRIM35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 19806-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 19806-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 19806-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U2OS using 19806-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM35 antibody (19806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	H9C2 cells, A431 cells, HEK-293 cells
Positive IHC detected in	human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19806-1-AP targets TRIM35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13584 Product name: Recombinant human TRIM35 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC018337 Sequence: MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRVRSLIAEERRNFLPTHQWIVTKTRLQTSSPNLQSRRQGQVQEHGACTAGEGQGLLGHAALL Predict reactive species
Full Name	tripartite motif-containing 35
Calculated Molecular Weight	57 kDa
Observed Molecular Weight	45~50 kDa
GenBank Accession Number	BC018337
Gene Symbol	TRIM35
Gene ID (NCBI)	23087
RRID	AB_3741968
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UPQ4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif 35 (TRIM35), also called hemopoietic lineage switch (Hls5), is a member of the RING finger, B box, coiled-coil (RBCC), or TRIM family E3 ligases expressed in a wide variety of hemopoietic cell types, including fetal liver progenitors. TRIM35 is reported to be a tumor suppressor gene and is expressed at low levels in several types of cancer (PMID: 34124276, 35081724). Western detected a band at 45-50 kDa possibly due to the high proline content.

Protocols

Product Specific Protocols
IF protocol for TRIM35 antibody 19806-1-AP	Download protocol
IHC protocol for TRIM35 antibody 19806-1-AP	Download protocol
WB protocol for TRIM35 antibody 19806-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TRIM35 Polyclonal antibody (catalog number 19806-1-AP) has 1 total citation. It appears in the journal Cell Signal, in a study titled "TRIM35 inhibits endometrial cancer progression via ubiquitination and degradation of EIF3E." The associated research field is cancer biology, specifically endometrial cancer, with the antibody applied in Western blot (WB) for human samples.