TRIM41 Polyclonal antibody

TRIM41 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18468-1-AP

Synonyms

tripartite motif containing 41, TRIM, RING finger-interacting protein with C kinase, RINCK, EC:2.3.2.27

---

Validation Data Gallery

• 

  WB analysis of BxPC-3 using 18468-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18468-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18468-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18468-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18468-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 18468-1-AP

  IP result of anti-TRIM41 (IP:18468-1-AP, 4ug; Detection:18468-1-AP 1:600) with human plasma lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18468-1-AP (TRIM41 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18468-1-AP (TRIM41 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18468-1-AP (TRIM41 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18468-1-AP (TRIM41 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of H9C2 using 18468-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TRIM41 antibody (18468-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: BxPC-3 cells, human placenta tissue, mouse liver tissue
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human skeletal muscle tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18468-1-AP targets TRIM41 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM41 fusion protein Ag13236
• Full Name: tripartite motif-containing 41
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 70-72 kDa
• GenBank Accession Number: BC018765
• Gene Symbol: TRIM41
• Gene ID (NCBI): 90933
• RRID: AB_10859823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Heliyon; IDI1 inhibits the cGAS-Sting signaling pathway in hepatocellular carcinoma; Authors - Lin Fu