TRIM44 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM44 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 11511-1-AP

Synonyms

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

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Validation Data Gallery

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  WB analysis of mouse testis using 11511-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11511-1-AP (TRIM44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11511-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11511-1-AP (TRIM44 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11511-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11511-1-AP (TRIM44 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human liver tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 17 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

11511-1-AP targets TRIM44 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM44 fusion protein Ag2070
• Full Name: tripartite motif-containing 44
• Calculated Molecular Weight: 344 aa, 38 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC024031
• Gene Symbol: TRIM44
• Gene ID (NCBI): 54765
• RRID: AB_2303802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense. This is a rabbit polyantibody raised against full length of human TRIM44.

Publications

Species	Application	Title
human	IHC	Gastroenterology; A 4-gene signature predicts survival of patients with resected adenocarcinoma of the esophagus, junction, and gastric cardia.; Authors - Peters Christopher J CJ
human	WB,IF	Autophagy; TRIM44 links the UPS to SQSTM1/p62-dependent aggrephagy and removing misfolded proteins.; Authors - Lin Lyu ; KO Validated
human	IP,WB,IF	Oncogene; TRIM44 mediated p62 deubiquitination enhances DNA damage repair by increasing nuclear FLNA and 53BP1 expression.; Authors - Lin Lyu ; KO Validated
human	WB	J Immunol; Novel function of Trim44 promotes an antiviral response by stabilizing VISA.; Authors - Yang Bo B
rat	WB,IHC,IF	Dis Model Mech; Cardiac-specific Trim44 knockout in rat attenuates isoproterenol-induced cardiac remodeling via inhibition of AKT/mTOR pathway.; Authors - Xiao-Yu Jiang
human	WB,IHC	Cancer Sci; TRIM44 promotes human esophageal cancer progression via the AKT/mTOR pathway.; Authors - Dian Xiong