TRIM45 Monoclonal antibody

TRIM45 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A7H11

Cat no : 66896-1-Ig

Synonyms

FLJ13181, RING finger protein 99, RNF99, TRIM45, tripartite motif containing 45

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Validation Data Gallery

• 

  WB analysis of HeLa using 66896-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66896-1-Ig (TRIM45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66896-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66896-1-Ig (TRIM45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66896-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66896-1-Ig (TRIM45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66896-1-Ig targets TRIM45 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM45 fusion protein Ag28300
• Full Name: tripartite motif-containing 45
• Calculated Molecular Weight: 562aa,62 kDa; 580aa,64 kDa
• Observed Molecular Weight: 62-64 kDa
• GenBank Accession Number: BC034943
• Gene Symbol: TRIM45
• Gene ID (NCBI): 80263
• RRID: AB_2882225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.