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TRIM46 Polyclonal antibody

TRIM46 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30298-1-AP

Synonyms

FLJ23229, Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM46 Polyclonal antibody (30298-1-AP)

    WB analysis using 30298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30298-1-AP (TRIM46 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM46 Polyclonal antibody (30298-1-AP)

    IF Staining of HepG2 using 30298-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (30298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30298-1-AP targets TRIM46 in WB, IF, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM46 fusion protein Ag33175
Full Name tripartite motif-containing 46
Calculated Molecular Weight 759 aa, 83 kDa
Observed Molecular Weight83 kDa
GenBank Accession NumberBC069416
Gene Symbol TRIM46
Gene ID (NCBI) 80128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

Protocols

Product Specific Protocols
WB protocol for TRIM46 antibody 30298-1-APDownload protocol
IF protocol for TRIM46 antibody 30298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Yannes (Verified Customer) (04-08-2024)

works well for western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: rat brain lysate, mouse primary hippocampal neurons