TRIM46 Recombinant monoclonal antibody, PBS Only

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  IF Staining of HepG2 using 83614-5-RR (same clone as 83614-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (83614-5-RR, Clone: 240557C2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83614-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83614-5-RR (same clone as 83614-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug TRIM46 Recombinant antibody (83614-5-RR, Clone:240557C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83614-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83614-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83614-5-RR against Human TRIM46 were performed. The affinity constant is 1.48 nM.

TRIM46 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83614-5-PBS

Clone No.240557C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

240557C2, GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46

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Product Information

83614-5-PBS targets TRIM46 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15290

  Product name: Recombinant human TRIM46 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC069416

  Sequence: MAEGEDMQTFTSIMDALVRISTSMKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSRRTLPKPDRLDRLLKSGFGTYPGRKRGALHPQVIMFPCPACQGDVELGERGLAGLFRNLTLERVVERYRQSVSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEEVTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLTYILGNQDTVQTQICELEEAVRHTEVSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLARLSAQI

• Full Name: tripartite motif-containing 46
• Calculated Molecular Weight: 759 aa, 83 kDa
• GenBank Accession Number: BC069416
• Gene Symbol: TRIM46
• Gene ID (NCBI): 80128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q7Z4K8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).