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  • KD/KO Validated

TRIM47 Polyclonal antibody

TRIM47 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 26885-1-AP

Synonyms

Tripartite motif-containing protein 47, tripartite motif containing 47, RNF100, GOA, EC:2.3.2.27



Tested Applications

Positive WB detected inU2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, mouse colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26885-1-AP targets TRIM47 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM47 fusion protein Ag22868
Full Name tripartite motif-containing 47
Calculated Molecular Weight 638 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC017299
Gene Symbol TRIM47
Gene ID (NCBI) 91107
RRIDAB_2880669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for TRIM47 antibody 26885-1-APDownload protocol
IHC protocol for TRIM47 antibody 26885-1-APDownload protocol
IP protocol for TRIM47 antibody 26885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasma

Trim47 overexpression correlates with poor prognosis in gastric cancer.

Authors - YuJian Xia
human,mouseWB,IHC,IP

Signal Transduct Target Ther

TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.

Authors - Yisong Qian
  • KD Validated
humanWB,IHC

In Vitro Cell Dev Biol Anim

TRIM47 promotes glioma angiogenesis by suppressing Smad4

Authors - Zifan Wang
  • KD Validated
human,mouseWB,IHC,IF

Clinics (Sao Paulo)

TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling

Authors - Xi Wang
  • KD Validated
ratWB

Exp Brain Res

Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats

Authors - Yingjun Zhu
  • KD Validated
humanWB,IF

Comput Struct Biotechnol J

Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress

Authors - Rui Su