Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, U2OS cells, mouse colon tissue, rat colon tissue |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human colon cancer tissue, human kidney tissue, mouse colon tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26885-1-AP targets TRIM47 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22868 Product name: Recombinant human TRIM47 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC017299 Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR Predict reactive species |
| Full Name | tripartite motif-containing 47 |
| Calculated Molecular Weight | 638 aa, 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC017299 |
| Gene Symbol | TRIM47 |
| Gene ID (NCBI) | 91107 |
| RRID | AB_2880669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96LD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| IP protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| WB protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM47 Polyclonal antibody (Cat# 26885-1-AP) has 25 citations across journals including Signal Transduction and Targeted Therapy, Acta Pharmaceutica Sinica B, Cell Death Discovery, Free Radical Biology and Medicine, Journal of Biological Chemistry, FASEB Journal, iScience, and Cancer Science. Research fields span oncology (hepatocellular, ovarian, gastric, glioma, nasopharyngeal, and cholangiocarcinoma), inflammation, neurology, metabolic reprogramming, sepsis, and toxicology, with applications in WB, IHC, IF, IP, and CoIP.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.
| ||
Acta Pharm Sin B GRK2 activates TRAF2-NF-κB signalling to promote hyperproliferation of fibroblast-like synoviocytes in rheumatoid arthritis | ||
Cell Death Discov Arginine methylation-dependent TRIM47 stability mediated by CARM1 promotes the metastasis of hepatocellular carcinoma | ||
Acta Pharmacol Sin Trim47 protects against hypoxic-ischaemic brain injury in neonatal rats by reducing brain microvascular endothelial inflammation and BBB disruption by interacting with ZO1.
| ||
Free Radic Biol Med TRIM47-CDO1 axis dictates hepatocellular carcinoma progression by modulating ferroptotic cell death through the ubiquitin‒proteasome system | ||
Cell Mol Life Sci TRIM47 drives metabolic reprogramming and tumor progression in Nasopharyngeal Carcinoma via K48-linked ubiquitination and degradation of SDHB. |
