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- KD/KO Validated
TRIM47 Polyclonal antibody
TRIM47 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 26885-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U2OS cells |
Positive IP detected in | U2OS cells |
Positive IHC detected in | human colon cancer tissue, human kidney tissue, mouse colon tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 7 publications below |
IHC | See 6 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Product Information
26885-1-AP targets TRIM47 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TRIM47 fusion protein Ag22868 |
Full Name | tripartite motif-containing 47 |
Calculated Molecular Weight | 638 aa, 70 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC017299 |
Gene Symbol | TRIM47 |
Gene ID (NCBI) | 91107 |
RRID | AB_2880669 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.
Protocols
Product Specific Protocols | |
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WB protocol for TRIM47 antibody 26885-1-AP | Download protocol |
IHC protocol for TRIM47 antibody 26885-1-AP | Download protocol |
IP protocol for TRIM47 antibody 26885-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.
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In Vitro Cell Dev Biol Anim TRIM47 promotes glioma angiogenesis by suppressing Smad4
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Clinics (Sao Paulo) TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling
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Exp Brain Res Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats
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Comput Struct Biotechnol J Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress |