TRIM47 Recombinant monoclonal antibody

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• TRIM47 Recombinant monoclonal antibody (86728-1-RR)

• 

  WB analysis using 86728-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of OVCAR-3 using 86728-1-RR

  OVCAR-3 cells were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86728-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86728-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86728-1-RR against Human TRIM47 were performed. The affinity constant is 1.77 nM.

TRIM47 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86728-1-RR

Clone No.251436A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

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Tested Applications

• Positive WB detected in: HeLa cells, mouse colon tissue, OVCAR-3 cells, U2OS cells, SMMC-7721 cells, PC-3 cells, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86728-1-RR targets TRIM47 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag22868

  Product name: Recombinant human TRIM47 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC017299

  Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR

• Full Name: tripartite motif-containing 47
• Calculated Molecular Weight: 638 aa, 70 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC017299
• Gene Symbol: TRIM47
• Gene ID (NCBI): 91107
• RRID: AB_3745084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96LD4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis. TRIM47, defined as E3 ubiquitin ligases, physically binds to SMAD4 and enhances the ubiquitination and degradation of SMAD4 (PMID: 39117713, 35513381).