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TRIM5 Polyclonal antibody

TRIM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 11476-1-AP


RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5

Tested Applications

Positive WB detected inMKN-45 cells, HeLa cells
Positive IHC detected inmouse liver tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11476-1-AP targets TRIM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM5 fusion protein Ag1973
Full Name tripartite motif-containing 5
Calculated Molecular Weight 493 aa, 56 kDa
Observed Molecular Weight 46 kDa, 70 kDa
GenBank Accession NumberBC021258
Gene Symbol TRIM5
Gene ID (NCBI) 85363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).


Product Specific Protocols
WB protocol for TRIM5 antibody 11476-1-APDownload protocol
IHC protocol for TRIM5 antibody 11476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols