TRIM55 Polyclonal antibody

TRIM55 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27274-1-AP

Synonyms

MURF 2, MuRF2, RING finger protein 29, RNF29, TRIM55, tripartite motif containing 55

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Validation Data Gallery

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  WB analysis using 27274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27274-1-AP (TRIM55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27274-1-AP (TRIM55 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 27274-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27274-1-AP (TRIM55 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM55 antibody (27274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: C2C12 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27274-1-AP targets TRIM55 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM55 fusion protein Ag25338
• Full Name: tripartite motif-containing 55
• Calculated Molecular Weight: 548 aa, 60 kDa
• Observed Molecular Weight: 60-65 kDa，75-80 kDa
• GenBank Accession Number: BC007750
• Gene Symbol: TRIM55
• Gene ID (NCBI): 84675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif-containing 55 (TRIM55), which is also called muscle-specific ring finger 2 (Murf2), has been indicated to participate in the natural development of muscle and the cardiovascular system. The expression of TRIM55 also inhibited several markers of cancer stem-like cells (PMID: 34974792). MURF2 mRNA studies revealed up to four potential isoforms generated by differential splicing events within the 3′-region of the gene: p60, p50, p27, and p60B MURF2 isoforms. The change in the ratio of E3 ligase isoforms regulates the sequential activation of proteasomal or autophagic degradation of target components(PMID: 15199100, PMID: 24124537). This antibody could detect multiple isoforms or post-translational modifications of TRIM55.