Tested Applications
| Positive WB detected in | C2C12 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27274-1-AP targets TRIM55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25338 Product name: Recombinant human TRIM55 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 265-452 aa of BC007750 Sequence: GIQFMDEPEMAVFLQNAKTLLKKISEASKAFQMEKIEHGYENMNHFTVNLNREEKIIREIDFYREDEDEEEEEGGEGEKEGEGEVGGEAVEVEEVENVQTEFPGEDENPEKASELSQVELQAAPGALPVSSPEPPPALPPAADAPVTQIGFEAPPLQGQAAAPASGSGADSEPARHIFSFSWLNSLNE Predict reactive species |
| Full Name | tripartite motif-containing 55 |
| Calculated Molecular Weight | 548 aa, 60 kDa |
| Observed Molecular Weight | 60-65 kDa，75-80 kDa |
| GenBank Accession Number | BC007750 |
| Gene Symbol | TRIM55 |
| Gene ID (NCBI) | 84675 |
| RRID | AB_3669590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYV6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tripartite motif-containing 55 (TRIM55), which is also called muscle-specific ring finger 2 (Murf2), has been indicated to participate in the natural development of muscle and the cardiovascular system. The expression of TRIM55 also inhibited several markers of cancer stem-like cells (PMID: 34974792). MURF2 mRNA studies revealed up to four potential isoforms generated by differential splicing events within the 3′-region of the gene: p60, p50, p27, and p60B MURF2 isoforms. The change in the ratio of E3 ligase isoforms regulates the sequential activation of proteasomal or autophagic degradation of target components(PMID: 15199100, PMID: 24124537). This antibody could detect multiple isoforms or post-translational modifications of TRIM55.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM55 antibody 27274-1-AP | Download protocol |
| IHC protocol for TRIM55 antibody 27274-1-AP | Download protocol |
| WB protocol for TRIM55 antibody 27274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
