Tested Applications
| Positive WB detected in | C2C12 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27274-1-AP targets TRIM55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25338 Product name: Recombinant human TRIM55 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 265-452 aa of BC007750 Sequence: GIQFMDEPEMAVFLQNAKTLLKKISEASKAFQMEKIEHGYENMNHFTVNLNREEKIIREIDFYREDEDEEEEEGGEGEKEGEGEVGGEAVEVEEVENVQTEFPGEDENPEKASELSQVELQAAPGALPVSSPEPPPALPPAADAPVTQIGFEAPPLQGQAAAPASGSGADSEPARHIFSFSWLNSLNE Predict reactive species |
| Full Name | tripartite motif-containing 55 |
| Calculated Molecular Weight | 548 aa, 60 kDa |
| Observed Molecular Weight | 60-65 kDa，75-80 kDa |
| GenBank Accession Number | BC007750 |
| Gene Symbol | TRIM55 |
| Gene ID (NCBI) | 84675 |
| RRID | AB_3669590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYV6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tripartite motif-containing 55 (TRIM55), which is also called muscle-specific ring finger 2 (Murf2), has been indicated to participate in the natural development of muscle and the cardiovascular system. The expression of TRIM55 also inhibited several markers of cancer stem-like cells (PMID: 34974792). MURF2 mRNA studies revealed up to four potential isoforms generated by differential splicing events within the 3′-region of the gene: p60, p50, p27, and p60B MURF2 isoforms. The change in the ratio of E3 ligase isoforms regulates the sequential activation of proteasomal or autophagic degradation of target components(PMID: 15199100, PMID: 24124537). This antibody could detect multiple isoforms or post-translational modifications of TRIM55.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM55 antibody 27274-1-AP | Download protocol |
| IHC protocol for TRIM55 antibody 27274-1-AP | Download protocol |
| WB protocol for TRIM55 antibody 27274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM55 Polyclonal antibody (Cat# 27274-1-AP) has 1 total citation. It was cited in Cancer Research (IF 22.6), in a study investigating how TRAPPC4 promotes GPX4 stability to drive ferroptosis resistance and tumor progression in head and neck squamous cell carcinoma. The antibody was used for Western blot in human samples, placing its cited application within the fields of oncology, ferroptosis biology, and head and neck cancer research.
| Species | Application | Title |
|---|---|---|
Cancer Res TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma |
