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TRIM6 Polyclonal antibody

TRIM6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11953-1-AP

Synonyms

RING finger protein 89, RNF89, TRIM6, tripartite motif containing 6



Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, human liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11953-1-AP targets TRIM6 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM6 fusion protein Ag2556
Full Name tripartite motif-containing 6
Calculated molecular weight 488 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC065575
Gene symbol TRIM6
Gene ID (NCBI) 117854
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIM6 antibody 11953-1-APDownload protocol
IHC protocol for TRIM6 antibody 11953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,CoIP

J Exp Clin Cancer Res

TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1.

Authors - Shuier Zheng
  • KD Validated