TRIM61 Polyclonal antibody

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  IHC staining of mouse liver using 24371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24371-1-AP (TRIM61 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 24371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM61 antibody (24371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TRIM61 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 24371-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

tripartite motif containing 61, TRIM, RNF35, RING finger protein 35

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Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24371-1-AP targets TRIM61 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21656

  Product name: Recombinant human TRIM61 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 176-209 aa of BC089393

  Sequence: MESPSVTRLECSCTISAHFNLRLPGSSDSSASGS

• Full Name: tripartite motif-containing 61
• Calculated Molecular Weight: 209 aa, 24 kDa
• GenBank Accession Number: BC089393
• Gene Symbol: TRIM61
• Gene ID (NCBI): 391712
• RRID: AB_3669434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5EBN2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM61 is a member of the tripartite motif (TRIM) protein family. The RING domain in TRIM proteins, including TRIM61, can mediate the conjugation of proteins with ubiquitin, small ubiquitin-like modifier (SUMO), or the ubiquitin-like molecule IFN-stimulated protein of 15 kDa (ISG15), contributing to the biological flexibility of TRIM proteins. The E3 ligase activity of TRIM proteins, such as TRIM61, is essential for their role in post-translational modification, which plays a crucial role in cellular processes often resulting in targeted degradation of conjugated proteins.