TRIM62 Polyclonal antibody

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  WB analysis using 15256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 15256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 15256-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 15256-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 15256-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

TRIM62 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15256-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tripartite motif-containing protein 62, tripartite motif containing 62, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM62

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Tested Applications

• Positive WB detected in: BGC-823 cells, SGC-7901 cells, mouse ovary tissue
• Positive IHC detected in: mouse liver tissue, rat ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15256-1-AP targets TRIM62 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7143

  Product name: Recombinant human TRIM62 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 126-475 aa of BC001222

  Sequence: TGIDDAFDELQRELKDQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTLDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

• Full Name: tripartite motif-containing 62
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC001222
• Gene Symbol: TRIM62
• Gene ID (NCBI): 55223
• RRID: AB_3669202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BVG3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Virol; TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.; Authors - Uchil Pradeep D PD