Tested Applications
| Positive WB detected in | BGC-823 cells, MCF-7 cells, SGC-7901 cells, mouse ovary tissue |
| Positive IHC detected in | mouse liver tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15256-1-AP targets TRIM62 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7143 Product name: Recombinant human TRIM62 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-475 aa of BC001222 Sequence: TGIDDAFDELQRELKDQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTLDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI Predict reactive species |
| Full Name | tripartite motif-containing 62 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC001222 |
| Gene Symbol | TRIM62 |
| Gene ID (NCBI) | 55223 |
| RRID | AB_3669202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVG3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM62 antibody 15256-1-AP | Download protocol |
| IHC protocol for TRIM62 antibody 15256-1-AP | Download protocol |
| WB protocol for TRIM62 antibody 15256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM62 Polyclonal antibody (Cat# 15256-1-AP) has a total of 1 citation. It appears in the Journal of Virology (J Virol), published in January 2013. The cited study investigates TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity, placing this antibody within the research fields of virology, innate immunity, and antiretroviral drug mechanisms.
| Species | Application | Title |
|---|---|---|
J Virol TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity. |
