TRIM69 Polyclonal antibody

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• KD/KO Validated

TRIM69 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12951-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

HSD 34, HSD34, RING finger protein 36, RNF36, Trif, TRIM69, tripartite motif containing 69

• 

  WB analysis of mouse testis using 12951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12951-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 12951-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12951-1-AP (TRIM69 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12951-1-AP targets TRIM69 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4074

  Product name: Recombinant human TRIM69 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-341 aa of BC033314

  Sequence: MEEELAIQQGQLETTLKELQTLRNMQKEAIAAHKENKLHLQQHVSMEFLKLHQFLHSKEKDILTELREEGKALNEEMELNLSQLQEQCLLAKDMLVSIQAKTEQQNSFDFLKDITTLLHSLEQGMKVLATRELISRKLNLGQYKGPIQYMVWREMQDTLCPGLSPLTLDPKTAHPNLVLSKSQTSVWHGDIKKIMPDDPERFDSSVAVLGSRGFTSGKWYWEVEVAKKTKWTVGVVRESIIRKGSCPLTPEQGFWLLRLRNQTDLKALDLPSFSLTLTNNLDKVGIYLDYEGGQLSFYNAKTMTHIYTFSNTFMEKLYPYFCPCLNDGGENKEPLHILHPQ

• Full Name: tripartite motif-containing 69
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC033314
• Gene Symbol: TRIM69
• Gene ID (NCBI): 140691
• RRID: AB_2208974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86WT6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E3 ubiquitin-protein ligase TRIM69 (TRIM69) plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis Indiana virus (PubMed:31375575, PubMed:31578292). It plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). It has 4 isoforms with molecular weights of 57, 39, 34, and 32 kDa.

Publications

What published studies show

The TRIM69 antibody (Cat# 12951-1-AP) has 8 total citations. Citations appear in Oncogene, Frontiers in Immunology, Mediators of Inflammation, Journal of Virology, Journal of Biomedical Research, Graefes Archive for Clinical and Experimental Ophthalmology, and Cell Physiology and Biochemistry. Research fields span gastric cancer metastasis, antiviral restriction (HIV-1, VSV, PRRSV), TLR-mediated inflammation, testis biology, UV-induced ferroptosis, and radioprotection.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Oncogene; TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD; Authors - Linqing Sun ; KD Validated
Human,monkey	WB	Front Immunol; DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection.; Authors - Huiyuan Jing
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
human	WB,IF	J Virol; Microtubule acetylation is not required for HIV-1 infection or TRIM69-mediated restriction of HIV-1 infection; Authors - Drew M Lichon
human	WB	J Virol; Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.; Authors - Tonya Kueck
mouse	IF, WB	J Biomed Res; The testis-specifically expressed gene Trim69 is not essential for fertility in mice.; Authors - Xi He ; KO Validated