Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22151-1-AP targets TRIM72 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17756 Product name: Recombinant human TRIM72 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-477 aa of BC033211 Sequence: MRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIISDDFKFQVWRKMFRALMPALEELTFDPSSAHPSLVVSSSGRRVECSEQKAPPAGEDPRQFDKAVAVVAHQQLSEGEHYWEVDVGDKPRWALGVIAAEAPRRGRLHAVPSQGLWLLGLREGKILEAHVEAKEPRALRSPERRPTRIGLYLSFGDGVLSFYDASDADALVPLFAFHERLPRPVYPFFDVCWHDKGKNAQPLLLVGPEGAEA Predict reactive species |
| Full Name | tripartite motif-containing 72 |
| Calculated Molecular Weight | 477 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC033211 |
| Gene Symbol | TRIM72 |
| Gene ID (NCBI) | 493829 |
| RRID | AB_2879009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZMU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM72 antibody 22151-1-AP | Download protocol |
| IP protocol for TRIM72 antibody 22151-1-AP | Download protocol |
| WB protocol for TRIM72 antibody 22151-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MG53 antibody (Cat# 22151-1-AP) has 16 citations published in journals including Nature Communications, Molecular Cancer, Theranostics, Free Radical Biology and Medicine, PLoS Pathogens, FASEB Journal, and others. Research fields span cardiovascular disease (cardiotoxicity, cardiac dysfunction, heart regeneration), oncology (colorectal cancer, melanoma), ferroptosis, alcoholic liver disease, spinal cord injury, viral infection, skeletal muscle biology, and wound healing.
| Species | Application | Title |
|---|---|---|
Mol Cancer LINC01852 inhibits the tumorigenesis and chemoresistance in colorectal cancer by suppressing SRSF5-mediated alternative splicing of PKM | ||
Nat Commun MG53 mediates skeletal muscle-liver cross-talk and enhances alcohol metabolism in alcoholic liver disease. | ||
Theranostics Mitochondria-encoded peptide MOTS-c participates in plasma membrane repair by facilitating the translocation of TRIM72 to membrane | ||
Int J Biol Macromol MG53/GMs/HA-Dex neural scaffold promotes the functional recovery of spinal cord injury by alleviating neuroinflammation | ||
Antioxidants (Basel) Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway |
