TRIM72 Polyclonal antibody

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  WB analysis using 22151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22151-1-AP (TRIM72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22151-1-AP

  IP result of anti-TRIM72 (IP:22151-1-AP, 4ug; Detection:22151-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IF Staining of mouse skeletal muscle using 22151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (22151-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (22151-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TRIM72 Polyclonal Antibody for WB, IF-P, IP, ELISA

Cat No. 22151-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

22151-1-AP targets TRIM72 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17756

  Product name: Recombinant human TRIM72 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 145-477 aa of BC033211

  Sequence: MRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIISDDFKFQVWRKMFRALMPALEELTFDPSSAHPSLVVSSSGRRVECSEQKAPPAGEDPRQFDKAVAVVAHQQLSEGEHYWEVDVGDKPRWALGVIAAEAPRRGRLHAVPSQGLWLLGLREGKILEAHVEAKEPRALRSPERRPTRIGLYLSFGDGVLSFYDASDADALVPLFAFHERLPRPVYPFFDVCWHDKGKNAQPLLLVGPEGAEA

• Full Name: tripartite motif-containing 72
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC033211
• Gene Symbol: TRIM72
• Gene ID (NCBI): 493829
• RRID: AB_2879009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZMU5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Int J Mol Sci; PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice; Authors - Baile Wu
mouse	WB	Int Immunopharmacol; MG53 binding to CAV3 facilitates activation of eNOS/NO signaling pathway to enhance the therapeutic benefits of bone marrow-derived mesenchymal stem cells in diabetic wound healing; Authors - Junwei Wu
human	WB,IP	Cancer Biomark; Ubiquitination and degradation of MGMT by TRIM72 increases the sensitivity of uveal melanoma cells to Dacarbazine treatment; Authors - Xun Li ; KD Validated
rat	WB	Biochem Biophys Res Commun; Mesenchymal stem cells-derived exosomes prevent sepsis-induced myocardial injury by a CircRTN4/miR-497-5p/MG53 pathway.; Authors - Jiang Li
rat	WB	BMC Cardiovasc Disord; Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner.; Authors - Jiawei Zhuang
human	WB	FASEB J; Chorionic villus-derived mesenchymal stem cells induce E3 ligase TRIM72 expression and regulate cell behaviors through ubiquitination of p53 in trophoblasts; Authors - Yan Li ; KD Validated