TRIM8 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27463-1-AP

Synonyms

GERP, RING finger protein 27, RNF27, TRIM8, tripartite motif containing 8

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Validation Data Gallery

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  WB analysis of L02 using 27463-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27463-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

27463-1-AP targets TRIM8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM8 fusion protein Ag26517
• Full Name: tripartite motif-containing 8
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC021925
• Gene Symbol: TRIM8
• Gene ID (NCBI): 81603
• RRID: AB_2880877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,IP	Oncogene; Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination; Authors - Lin Wang ; KD Validated
human	WB	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu
human	WB,IF	Cancer Med; TRIM8 promotes ovarian cancer proliferation and migration by targeting VDAC2 for ubiquitination and degradation; Authors - Fei Wu ; KD Validated
human	WB,IHC	J Orthop Surg Res; The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma; Authors - Jian Wen
human	WB	Exp Mol Pathol; Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis.; Authors - Lei Li
human	WB	Adipocyte; Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway; Authors - Mingxue Zhu ; KD Validated