TRIM9 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 10786-1-AP

Synonyms

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

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Validation Data Gallery

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  WB analysis using 10786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10786-1-AP (TRIM9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10786-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10786-1-AP (TRIM9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10786-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10786-1-AP (TRIM9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10786-1-AP

  IP result of anti-TRIM9 (IP:10786-1-AP, 4ug; Detection:10786-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat brain using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10786-1-AP (TRIM9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10786-1-AP (TRIM9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat brain tissue, human prostate cancer tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

10786-1-AP targets TRIM9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM9 fusion protein Ag1238
• Full Name: tripartite motif-containing 9
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 79 kDa, 90 kDa, 61 kDa
• GenBank Accession Number: BC013414
• Gene Symbol: TRIM9
• Gene ID (NCBI): 114088
• RRID: AB_2209130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.

Publications

Species	Application	Title
human	WB	Cell Res; PMID: 26915459; Authors - Yunfei Qin ; KD Validated
human, mouse, rat	WB	Neurobiol Dis; TRIM9, a novel brain-specific E3 ubiquitin ligase, is repressed in the brain of Parkinson's disease and dementia with Lewy bodies.; Authors - Tanji Kunikazu K
rat	WB,IP	Mol Med Rep; Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.; Authors - Wenjun Zhao ; KD Validated
human	IHC	Springerplus; Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patients.; Authors - Chieko Mishima
mouse	WB	Elife; A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway; Authors - R Samuel Herron
mouse	WB	Mol Biol Cell; The E3 ubiquitin ligase TRIM9 regulates synaptic function and actin dynamics in response to netrin-1; Authors - Laura E McCormick ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Echo (Verified Customer) (03-20-2024)

The quality was good. Saw two bands for western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TRIM9 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10786-1-AP)