Tested Applications
| Positive WB detected in | U2OS cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31976-1-AP targets TRIML2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37106 Product name: Recombinant human TRIML2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-155 aa of BC111959 Sequence: MVCGIQEAAENYRKLFQEILNTSREKLEAAKSILTDEQERMAMIQEEEQNFKKMIESEYSMRLRLLNEECEQNLQRQQECISDLNLRETLLNQAIKLATELEEMFQEMLQRLGRVGRENMEKLKESEARASEQVRSLLKLIVELEKKCGEGTLAL Predict reactive species |
| Full Name | tripartite motif family-like 2 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC111959 |
| Gene Symbol | TRIML2 |
| Gene ID (NCBI) | 205860 |
| RRID | AB_3670164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N7C3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tripartite motif family like 2 (TRIML2, also known as SPRYD6) is a member of the tripartite motif (TRIM) family of proteins. It probably interacts with p53 to facilitate the modification of p53 with small ubiquitin-like modifier 2 (SUMO2) (PMID: 25256710). By regulating p53, TRIML2 may also be an important factor in the pathogenesis of Alzheimer's disease (PMID: 27471163).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIML2 antibody 31976-1-AP | Download protocol |
| WB protocol for TRIML2 antibody 31976-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIML2 Polyclonal antibody (Cat# 31976-1-AP) has a total of 1 citation. It appears in the Journal of Translational Medicine (IF 9.7), published in November 2025. The cited study investigates TRIML2's role in promoting malignant progression of head and neck squamous cell carcinoma through canonical Wnt signaling and tumor immune escape. The associated research fields include cancer biology, oncogenic signaling pathways, and tumor immunology. The antibody was validated in human samples using WB and IHC applications.
| Species | Application | Title |
|---|---|---|
J Transl Med TRIML2 promotes malignant progression of head and neck squamous cell carcinoma via canonical Wnt signaling and tumor immune escape.
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