TRIOBP Polyclonal antibody

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  WB analysis using 16124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16124-1-AP (TRIOBP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 16124-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16124-1-AP (TRIOBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 16124-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using TARA antibody (16124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TRIOBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16124-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Trio-associated repeat on actin, TRIO and F-actin-binding protein, TRF1-associated protein of 68 kDa, TARA, HRIHFB2122

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 8 publications below

Product Information

16124-1-AP targets TRIOBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9092

  Product name: Recombinant human TARA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 5-379 aa of BC013278

  Sequence: EATDSRTPEVPAGEGPRRGLGAPLTEDQQNRLSEEIEKKWQELEKLPLRENKRVPLTALLNQSRGERRGPPSDGHEALEKEVQALRAQLEAWRLQGEAPQSALRSQEDGHIPPGYISQEACERSLAEMESSHQQVMEELQRHHERELQRLQQEKEWLLAEETAATASAIEAMKKAYQEELSRELSKTRSLQQGPDGLRKQHQSDVEALKRELQVLSEQYSQKCLEIGALMRQAEEREHTLRRCQQEGQELLRHNQELHGRLSEEIDQLRGFIASQGMGNGCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDELQMMQKDKRFTSGKYQDVYVELSHIKTRSEREIEQLKEHLRLAMAALQEKESMRNSLAE

• Full Name: TRIO and F-actin binding protein
• Calculated Molecular Weight: 2356 aa, 261 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC013278
• Gene Symbol: TRIOBP
• Gene ID (NCBI): 11078
• RRID: AB_2209237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2D6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Proc Natl Acad Sci U S A; Unbalanced bidirectional radial stiffness gradients within the organ of Corti promoted by TRIOBP.; Authors - Hesam Babahosseini
human	IF	J Cell Biol; ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points.; Authors - Jocelyn F Krey
mouse	IF	Mol Neurobiol; Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.; Authors - Qingxiu Yao
mouse	IF	J Cell Sci; GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region; Authors - Paroma Chatterjee
mouse	IF	Elife; Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB; Authors - Ling-Yun Zhou
human,mouse	WB	Cell Mol Life Sci; TRIOBP modulates β-catenin signaling by regulation of miR-29b in idiopathic pulmonary fibrosis; Authors - Lan Wang