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  • KD/KO Validated

TRIP10 Polyclonal antibody

TRIP10 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10798-1-AP

Synonyms

Cdc42 interacting protein 4, CIP4, HSTP, Protein Felic, Salt tolerant protein, STOT, STP, TR interacting protein 10, TRIP 10, TRIP10



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, BxPC-3 cells, mouse skeletal muscle tissue
Positive IP detected inK-562 cells
Positive IF detected inBxPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10798-1-AP targets TRIP10 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIP10 fusion protein Ag1251
Full Name thyroid hormone receptor interactor 10
Calculated molecular weight 63 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC013002
Gene symbol TRIP10
Gene ID (NCBI) 9322
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIP10 antibody 10798-1-APDownload protocol
IF protocol for TRIP10 antibody 10798-1-APDownload protocol
IP protocol for TRIP10 antibody 10798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Exp Clin Cancer Res

CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.

Authors - Dong-Fang Meng
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects human version of the protein in several cell types. 293T, U2OS and A549 cells tested. Antibody also IP's the endogenous protein

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
TRIP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in 293T (Cat no:10798-1-AP)