TRIP10 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIP10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 10798-1-AP

Synonyms

TRIP-10, TR-interacting protein 10, Thyroid receptor-interacting protein 10, hSTP, CIP4

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Validation Data Gallery

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  WB analysis using 10798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10798-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10798-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10798-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10798-1-AP

  IP result of anti-TRIP10 (IP:10798-1-AP, 4ug; Detection:10798-1-AP 1:10000) with K-562 cells lysate 1760 ug.

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  IF Staining of BxPC-3 using 10798-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIP10 antibody (10798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10798-1-AP

  Immunofluorescent analysis of HeLa cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, BxPC-3 cells, mouse skeletal muscle tissue, HeLa cells, NIH/3T3 cells, C6 cells
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: HeLa cells, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10798-1-AP targets TRIP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIP10 fusion protein Ag1251
• Full Name: thyroid hormone receptor interactor 10
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC013002
• Gene Symbol: TRIP10
• Gene ID (NCBI): 9322
• RRID: AB_2209248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins.; Authors - Shinya Yuge
human	WB,IHC	J Exp Clin Cancer Res; CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.; Authors - Dong-Fang Meng ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects human version of the protein in several cell types. 293T, U2OS and A549 cells tested. Antibody also IP's the endogenous protein

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

TRIP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in 293T (Cat no:10798-1-AP)