Validation Data Gallery
|Positive WB detected in||HEK-293 cells, K-562 cells, BxPC-3 cells, mouse skeletal muscle tissue|
|Positive IP detected in||K-562 cells|
|Positive IF detected in||BxPC-3 cells, HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10798-1-AP targets TRIP10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRIP10 fusion protein Ag1251|
|Full Name||thyroid hormone receptor interactor 10|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||75-85 kDa|
|GenBank accession number||BC013002|
|Gene ID (NCBI)||9322|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
anwesh (Verified Customer) (04-01-2019)
Great antibody. Detects human version of the protein in several cell types. 293T, U2OS and A549 cells tested. Antibody also IP's the endogenous protein