TRIP12 Polyclonal antibody

• 

  WB analysis of MCF-7 using 25303-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25303-1-AP (TRIP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

TRIP12 Polyclonal Antibody for WB, ELISA

Cat No. 25303-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, CoIP, ELISA

Synonyms

KIAA0045, TR interacting protein 12, TRIP 12, TRIP12

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Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

25303-1-AP targets TRIP12 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18112

  Product name: Recombinant human TRIP12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1691-2040 aa of BC114556

  Sequence: QRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

• Full Name: thyroid hormone receptor interactor 12
• Calculated Molecular Weight: 1992 aa, 220 kDa
• Observed Molecular Weight: 190-220 kDa
• GenBank Accession Number: BC114556
• Gene Symbol: TRIP12
• Gene ID (NCBI): 9320
• RRID: AB_2880020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14669
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Combinatorial ubiquitin code degrades deubiquitylation-protected substrates; Authors - Mai Morita
human	WB	Mol Cell; TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.; Authors - Ai Kaiho-Soma ; KO Validated
human	WB	Clin Epigenetics; EZH2 K63-polyubiquitination affecting migration in extranodal natural killer/T-cell lymphoma; Authors - Boheng Li ; KD Validated
human	WB,IP,CoIP	Oncogene; Engineered targeting OIP5 sensitizes bladder cancer to chemotherapy resistance via TRIP12-PPP1CB-YBX1 axis; Authors - Xianteng Wang
	WB	Nat Commun; Intrinsic signaling pathways modulate targeted protein degradation; Authors - Yuki Mori
	WB	bioRxiv; Dynamic regulation of the oxidative stress response by the E3 ligase TRIP12; Authors - Andrew J Ingersoll