Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25303-1-AP targets TRIP12 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18112 Product name: Recombinant human TRIP12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1691-2040 aa of BC114556 Sequence: QRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS Predict reactive species |
| Full Name | thyroid hormone receptor interactor 12 |
| Calculated Molecular Weight | 1992 aa, 220 kDa |
| Observed Molecular Weight | 190-220 kDa |
| GenBank Accession Number | BC114556 |
| Gene Symbol | TRIP12 |
| Gene ID (NCBI) | 9320 |
| RRID | AB_2880020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14669 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIP12 antibody 25303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TRIP12 antibody (Cat# 25303-1-AP) has 12 citations spanning journals including Nature Communications, Molecular Cell, Cell Discovery, Cell Reports, Oncogene, Clinical Epigenetics, Journal of Virology, and bioRxiv. Research fields covered include ubiquitin-mediated protein degradation, targeted protein degradation (TPD), oxidative stress response, cancer (bladder, lymphoma, solid tumors), epigenetic regulation, cuproptosis resistance, and HIV-1 replication. Applications are predominantly Western blot, with some IP/Co-IP and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeted degradation of USP7 in solid cancer cells reveals distinct effects of deubiquitinase degraders and inhibitors. | ||
Cell Discov NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance | ||
Mol Cell Deubiquitinases cleave ubiquitin-fused ribosomal proteins and physically counteract their targeting to the UFD pathway. | ||
Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.
|
Reviews
What customers say
One reviewer (5/5) used this antibody at 1:1000 dilution for Western Blot and Immunofluorescence on U2OS, HeLa, and MDA-MB-231 cells. They reported it was the only brand that worked after trying three different options, praising its overall performance. Their sole concern was a nonspecific band appearing above the 250 kDa mark, but they still found it highly effective for their applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Evra (Verified Customer) (09-04-2026) | We've tried three different brands, and this is the only one that works. My only gripe is that there appears to be a nonspecific band above the 250kDa mark. But other than that, it works really well.
 |
