TRIT1 Polyclonal antibody

TRIT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17141-1-AP

Synonyms

hGRO1, IPP transferase, IPPT, IPT, IPTase, MOD5, TRIT1, tRNA isopentenyltransferase, tRNA isopentenyltransferase 1

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Validation Data Gallery

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  WB analysis using 17141-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 17141-1-AP (TRIT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17141-1-AP targets TRIT1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIT1 fusion protein Ag10906
• Full Name: tRNA isopentenyltransferase 1
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 53-60 kDa
• GenBank Accession Number: BC107569
• Gene Symbol: TRIT1
• Gene ID (NCBI): 54802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IPTases (TRIT1) belong to a group of enzymes that modify both cytoplasmic and mitochondrial tRNAs of eukaryotic cells. Pathogenic mutations to human IPTase (TRIT1) cause mitochondrial insufficiency and neurodevelopmental disease, principally due to decreased modification of the mt-tRNA substrates.(PMID: 32324744, PMID: 36049610)