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TRMT1 Polyclonal antibody, PBS Only (14970-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 14970-1-AP (same clone as 14970-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14970-1-AP (TRMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 14970-1-AP (same clone as 14970-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 14970-1-AP (same clone as 14970-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 14970-1-AP (same clone as 14970-1-PBS)
Immunofluorescent analysis of HeLa cells using 14970-1-AP (TRMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.

TRMT1 Polyclonal antibody, PBS Only

Name: TRMT1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14970-1-PBS

TRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14970-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14970-1-PBS targets TRMT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6837 Product name: Recombinant human TRMT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 371-659 aa of BC002492 Sequence: KFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID Predict reactive species
Full Name	TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
Calculated Molecular Weight	81 kDa
Observed Molecular Weight	72 kDa
GenBank Accession Number	BC002492
Gene Symbol	TRMT1
Gene ID (NCBI)	55621
RRID	AB_2209504
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NXH9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.