TRMT11 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRMT11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17555-1-AP

Synonyms

tRNA guanosine-2'-O-methyltransferase TRM11 homolog, tRNA (guanine(10)-N2)-methyltransferase homolog, TRM11, MDS024, EC:2.1.1.-

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Validation Data Gallery

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  WB analysis using 17555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17555-1-AP

  WB result of TRMT11 antibody (17555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT11 transfected HEK-293 cells.

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  WB analysis of LNCaP using 17555-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17555-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17555-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17555-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17555-1-AP (TRMT11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17555-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17555-1-AP (TRMT11 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17555-1-AP

  Immunofluorescent analysis of HepG2 cells, using IFT52 antibody 17555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: PC-3 cells, HEK-293 cells, LNCaP cells, K-562 cells, mouse testis tissue, HepG2 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17555-1-AP targets TRMT11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRMT11 fusion protein Ag11717
• Full Name: tRNA methyltransferase 11 homolog (S. cerevisiae)
• Calculated Molecular Weight: 463 aa, 53 kDa
• Observed Molecular Weight: 48-53 kDa
• GenBank Accession Number: BC056878
• Gene Symbol: TRMT11
• Gene ID (NCBI): 60487
• RRID: AB_2209511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Microbiol Immunol; BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.; Authors - Bea Clarise B Garcia