TRMT6 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83843-6-RR (same clone as 83843-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83843-6-RR (TRMT6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83843-6-RR (same clone as 83843-6-PBS)

  WB result of TRMT6 antibody (83843-6-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells. This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83843-6-RR (same clone as 83843-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-6-RR (TRMT6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83843-6-RR (same clone as 83843-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-6-RR, Clone: 240911D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83843-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83843-6-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

TRMT6 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83843-6-PBS

Clone No.240911D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240911D3, CGI 09, CGI-09, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, TRM6

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Product Information

83843-6-PBS targets TRMT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10176

  Product name: Recombinant human TRMT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-497 aa of BC001262

  Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS

• Full Name: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC001262
• Gene Symbol: TRMT6
• Gene ID (NCBI): 51605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UJA5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR.