TRMT61A Polyclonal antibody

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  WB analysis using 31169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31169-1-AP (TRMT61A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 31169-1-AP

  WB result of TRMT61A antibody (31169-1-AP; 1:3000; room temperature for 1.5 hours) with wild-type and TRMT61A knockout RAW 264.7 cells.

• Featured Product
• KD/KO Validated

TRMT61A Polyclonal Antibody for WB, ELISA

Cat No. 31169-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

hTRM61, Gcd14p, GCD14, EC:2.1.1.220, EC:2.1.1.-

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Tested Applications

• Positive WB detected in: 5637 cells, RAW 264.7 cells, MCF-7 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31169-1-AP targets TRMT61A in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRMT61A fusion protein Ag34396
• Full Name: tRNA methyltransferase 61 homolog A (S. cerevisiae)
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC010167
• Gene Symbol: TRMT61A
• Gene ID (NCBI): 115708
• RRID: AB_3669880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96FX7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N1-methyladenosine (m1A) is an important posttranscriptional modification and occurs throughout the tRNA structure.m1A at position 58 (m1A58) is the predominant m1A modification of cytoplasmic tRNA in eukaryotic cells. The formation of m1A58 on cytoplasmic tRNAs is mediated by the methyltransferase complex TRMT6/TRMT61A, in which TRMT61A functions as the catalytic subunit and TRMT6 is responsible for tRNA binding.