TRMT61B Polyclonal antibody

TRMT61B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26009-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

EC:2.1.1.-, EC:2.1.1.220, mRNA methyladenosine-N(1)-methyltransferase, tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis of HepG2 using 26009-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26009-1-AP (TRMT61B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 26009-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26009-1-AP (TRMT61B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 26009-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26009-1-AP (TRMT61B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 26009-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26009-1-AP (TRMT61B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HepG2 cells, HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

26009-1-AP targets TRMT61B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23307 Product name: Recombinant human TRMT61B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-477 aa of BC010365 Sequence: QSATEVEERHVSPSCSTSRERPFQAGELILAETGEGETKFKKLFRLNNFGLLNSNWGAVPFGKIVGKFPGQILRSSFGKQYMLRRPALEDYVVLMKRGTAITFPKDINMILSMMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVRKDHHDLAKKNYKHWRDSWKLSHVEEWPDNVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTLPVFYPHLKHGGVCAVYVVNITQVIELLDGIRTCELALSCEKISEVIVRDWLVCLAKQKNGILAQKVESKINTDVQLDSQEKIGVKGELFQEDDHEESHSDFPYGSFPYVARPVHWQPGHTAFLVKLRKVKPQLN Predict reactive species
Full Name	tRNA methyltransferase 61 homolog B (S. cerevisiae)
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC010365
Gene Symbol	TRMT61B
Gene ID (NCBI)	55006
RRID	AB_2880333
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BVS5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for TRMT61B antibody 26009-1-AP	Download protocol
WB protocol for TRMT61B antibody 26009-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. Authors - Uwe Richter View Article
human	WB	Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation Authors - Liang Wang View Article
human	WB	Cell Discov ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis. Authors - Wenjun Kuang View Article
	WB	Cancer Lett YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer Authors - Hongyu Chen View Article