TRMT61B Polyclonal antibody

TRMT61B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26009-1-AP

Synonyms

TRMT61B

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Validation Data Gallery

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  WB analysis of HepG2 using 26009-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26009-1-AP (TRMT61B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26009-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26009-1-AP (TRMT61B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26009-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26009-1-AP (TRMT61B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26009-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26009-1-AP (TRMT61B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

26009-1-AP targets TRMT61B in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRMT61B fusion protein Ag23307
• Full Name: tRNA methyltransferase 61 homolog B (S. cerevisiae)
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC010365
• Gene Symbol: TRMT61B
• Gene ID (NCBI): 55006
• RRID: AB_2880333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
human	WB	Cell Discov; ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.; Authors - Wenjun Kuang