TRNT1 Polyclonal antibody

TRNT1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25376-1-AP

Synonyms

MTO2, MTO2 homolog, MTU1, TRMT, TRMT1, TRMU, TRNT1

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Validation Data Gallery

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  IHC staining of mouse skeletal muscle using 25376-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 25376-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25376-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25376-1-AP (TRMU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25376-1-AP targets TRNT1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRNT1 fusion protein Ag21930
• Full Name: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
• Calculated Molecular Weight: 421 aa, 48 kDa
• GenBank Accession Number: BC027991
• Gene Symbol: TRMU
• Gene ID (NCBI): 55687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.