TRNP1 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83410-3-RR (same clone as 83410-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83410-3-RR (TRNP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83410-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83410-3-RR (same clone as 83410-3-PBS)

  WB result of TRNP1 antibody (83410-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRNP1 transfected HeLa cells. This data was developed using the same antibody clone with 83410-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83410-3-RR (same clone as 83410-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-3-RR, Clone:240331C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83410-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00432-1

  Cytometric bead array standard curve of MP00432-1, TRNP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83410-3-PBS. Detection antibody: 83410-2-PBS. Standard: Ag22504. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00432-3

  Cytometric bead array standard curve of MP00432-3, TRNP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83410-3-PBS. Detection antibody: 83410-1-PBS. Standard: Ag22504. Range: 1.25-80 ng/mL.

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  Affinity and Kinetic Characterization of 83410-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83410-3-RR against Human TRNP1 were performed. The affinity constant is 72.8 pM.

• Featured Product
• KD/KO Validated

TRNP1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83410-3-PBS

Clone No.240331C6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

240331C6, C1orf225, TMF-regulated nuclear protein 1, TNRP, TRNP

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Product Information

83410-3-PBS targets TRNP1 as part of a matched antibody pair:

MP00432-1: 83410-3-PBS capture and 83410-2-PBS detection (validated in Cytometric bead array)

MP00432-3: 83410-3-PBS capture and 83410-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRNP1 fusion protein Ag22504
• Full Name: TMF1-regulated nuclear protein 1
• Calculated Molecular Weight: 227 aa, 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC069216
• Gene Symbol: TRNP1
• Gene ID (NCBI): 388610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6NT89
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.