TROAP Polyclonal antibody

• Home
• Products
• Primary Antibodies
• TROAP Polyclonal antibody (13634-1-AP)

• Featured Product
• KD/KO Validated

TROAP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13634-1-AP Copied!

Publications (15)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Tastin, Trophinin assisting protein, Trophinin associated protein, Trophinin-assisting protein, Trophinin-associated protein

• 

  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for TROAP in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, PC-3 cells, HT-29 cells, Jurkat cells
• Positive IHC detected in: human gliomas tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13634-1-AP targets TROAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4562

  Product name: Recombinant human TROAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 428-778 aa of BC032583

  Sequence: QEVKIQRIGILQQLLRQEVEGLVGGQCVPLNGGSSLDMVELQPLLTEISRTLNATEHNSGTSHLPGLLKHSGLPKPCLPEECGEPQPCPPAEPGPPEAFCRSEPEIPEPSLQEQLEVPEPYPPAEPRPLESCCRSEPEIPESSRQEQLEVPEPCPPAEPRPLESYCRIEPEIPESSRQEQLEVPEPCPPAEPGPLQPSTQGQSGPPGPCPRVELGASEPCTLEHRSLESSLPPCCSQWAPATTSLIFSSQHPLCASPPICSLQSLRPPAGQAGLSNLAPRTLALRERLKSCLTAIHCFHEARLDDECAFYTSRAPPSGPTRVCTNPVATLLEWQDALCFIPVGSAAPQGSP

• Full Name: trophinin associated protein (tastin)
• Calculated Molecular Weight: 778 aa, 84 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032583
• Gene Symbol: TROAP
• Gene ID (NCBI): 10024
• RRID: AB_10597857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12815
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).

Publications

What published studies show

The anti-TROAP antibody (Cat# 13634-1-AP) has 15 citations published in journals including MedComm, Dev Cell, Biomed Pharmacother, Brief Bioinform, CNS Neurosci Ther, Med Oncol, Oncol Rep, Funct Integr Genomics, Cell Cycle, Mol Carcinog, Front Oncol, J Cancer, and J Cancer Res Clin Oncol. Research fields span multiple cancer types (colorectal, glioma, lung, prostate, gastric, ovarian, renal, esophageal), tumor microenvironment, Wnt/β-Catenin and PI3K/AKT signaling, EMT, and HCMV assembly.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	MedComm (2020); Integrative analysis of TROAP with molecular features, carcinogenesis, and related immune and pharmacogenomic characteristics in soft tissue sarcoma; Authors - Chao Tu ; KD Validated
		Dev Cell; The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.; Authors - Dean J Procter
human	WB	Biomed Pharmacother; MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer.; Authors - Xiaoyong Ye ; KD Validated
human	IHC	Brief Bioinform; Comprehensive assessment of cellular senescence in the tumor microenvironment.; Authors - Xiaoman Wang
human	WB	CNS Neurosci Ther; TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells.; Authors - Zong-Qing Zhao
human	WB	Oncol Rep; TROAP regulates prostate cancer progression via the WNT3/survivin signalling pathways.; Authors - Jianqing Ye ; KD Validated