TROAP Polyclonal antibody

• Featured Product
• KD/KO Validated

TROAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13634-1-AP

Synonyms

TASTIN, TROAP, Trophinin assisting protein, Trophinin associated protein

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Validation Data Gallery

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  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, PC-3 cells, HT-29 cells, Jurkat cells
• Positive IHC detected in: human gliomas tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 5 publications below

Product Information

13634-1-AP targets TROAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TROAP fusion protein Ag4562
• Full Name: trophinin associated protein (tastin)
• Calculated Molecular Weight: 778 aa, 84 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032583
• Gene Symbol: TROAP
• Gene ID (NCBI): 10024
• RRID: AB_10597857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).

Publications

Species	Application	Title
		Dev Cell; The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.; Authors - Dean J Procter
human	IHC	Brief Bioinform; Comprehensive assessment of cellular senescence in the tumor microenvironment.; Authors - Xiaoman Wang
human	WB	Biomed Pharmacother; MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer.; Authors - Xiaoyong Ye ; KD Validated
human	WB	Cell Cycle; MiR-532-3p suppresses cell viability, migration and invasion of clear cell renal cell carcinoma through targeting TROAP.; Authors - Bin Gao
human	WB	Front Oncol; Downregulation of Methyltransferase-Like 14 Promotes Ovarian Cancer Cell Proliferation Through Stabilizing TROAP mRNA.; Authors - Yize Li
human	WB	CNS Neurosci Ther; TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells.; Authors - Zong-Qing Zhao