TROAP Recombinant monoclonal antibody

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• TROAP Recombinant monoclonal antibody (86735-2-RR)

• 

  WB analysis using 86735-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86735-2-RR (TROAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of SK-BR-3 using 86735-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SK-BR-3 cells using TROAP antibody (86735-2-RR, Clone: 251538B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86735-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86735-2-RR against Human TROAP were performed. The affinity constant is 89.2 pM.

TROAP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86735-2-RR

Clone No.251538B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Tastin, Trophinin assisting protein, Trophinin associated protein, Trophinin-assisting protein, Trophinin-associated protein

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, PANC-1 cells, SK-BR-3 cells
• Positive IF/ICC detected in: SK-BR-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86735-2-RR targets TROAP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4562

  Product name: Recombinant human TROAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 428-778 aa of BC032583

  Sequence: QEVKIQRIGILQQLLRQEVEGLVGGQCVPLNGGSSLDMVELQPLLTEISRTLNATEHNSGTSHLPGLLKHSGLPKPCLPEECGEPQPCPPAEPGPPEAFCRSEPEIPEPSLQEQLEVPEPYPPAEPRPLESCCRSEPEIPESSRQEQLEVPEPCPPAEPRPLESYCRIEPEIPESSRQEQLEVPEPCPPAEPGPLQPSTQGQSGPPGPCPRVELGASEPCTLEHRSLESSLPPCCSQWAPATTSLIFSSQHPLCASPPICSLQSLRPPAGQAGLSNLAPRTLALRERLKSCLTAIHCFHEARLDDECAFYTSRAPPSGPTRVCTNPVATLLEWQDALCFIPVGSAAPQGSP

• Full Name: trophinin associated protein (tastin)
• Calculated Molecular Weight: 778 aa, 84 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC032583
• Gene Symbol: TROAP
• Gene ID (NCBI): 10024
• RRID: AB_3745091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12815
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).