TRPA1 Recombinant monoclonal antibody

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  WB analysis using 84986-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84986-3-RR (TRPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84986-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84986-3-RR (TRPA1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 84986-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84986-3-RR (TRPA1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRPA1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84986-3-RR

Clone No.250625D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ANKTM1, p120, Wasabi receptor

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, U2OS cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84986-3-RR targets TRPA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: transient receptor potential cation channel, subfamily A, member 1
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_007332
• Gene Symbol: TRPA1
• Gene ID (NCBI): 8989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75762
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds.