TRPC1 Polyclonal antibody

TRPC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24727-1-AP

Synonyms

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

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Validation Data Gallery

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  WB analysis using 24727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24727-1-AP (TRPC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24727-1-AP (TRPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 24727-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24727-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24727-1-AP targets TRPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPC1 fusion protein Ag16488
• Full Name: transient receptor potential cation channel, subfamily C, member 1
• Calculated Molecular Weight: 793 aa, 91 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC113953
• Gene Symbol: TRPC1
• Gene ID (NCBI): 7220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.