TRPC3 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HEK-293 using 84019-5-RR (same clone as 84019-5-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84019-5-RR (TRPC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84019-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 84019-5-RR (same clone as 84019-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84019-5-RR (TRPC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84019-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00972-1

  Cytometric bead array standard curve of MP00972-1, TRPC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84019-5-PBS. Detection antibody: 84019-4-PBS. Standard: Ag13024. Range: 0.781-100 ng/mL.

TRPC3 {ptg:Brand} Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84019-5-PBS

Clone No.241075H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Transient receptor protein 3, Short transient receptor potential channel 3, hTrp-3, hTrp3, hTrp 3

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Product Information

84019-5-PBS targets TRPC3 as part of a matched antibody pair:

MP00972-1: 84019-5-PBS capture and 84019-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRPC3 fusion protein Ag13024
• Full Name: transient receptor potential cation channel, subfamily C, member 3
• Calculated Molecular Weight: 848 aa, 97 kDa
• Observed Molecular Weight: 105 kDa, 97 kDa
• GenBank Accession Number: BC093682
• Gene Symbol: TRPC3
• Gene ID (NCBI): 7222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13507
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TRPC3 (Short transient receptor potential channel 3), also known as TRP3. It is expected to be located in the cell membrane, which is expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis. TRPC3 is one of the classical members of the mammalian transient receptor potential (TRP) superfamily of ion channels. TRPC3 is a molecule with intriguing sensory features including the direct recognition of and activation by diacylglycerols (DAG). Although TRPC3 channels are ubiquitously expressed, they appear to control functions of the cardiovascular system and the brain in a highly specific manner. Moreover, a role of TRPC3 in immunity, cancer, and tissue remodeling has been proposed, generating much interest in TRPC3 as a target for pharmacological intervention. The molecular weight of TRPC3 is 105 kDa, and it can recognize another isoform. (PMID: 30037143)