TRPC4 Polyclonal antibody

TRPC4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21349-1-AP

Synonyms

hTrp 4, HTRP4, Trp related protein 4, TRP4, TRPC4



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21349-1-AP is TRPC4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC4 fusion protein Ag15698
Full Name transient receptor potential cation channel, subfamily C, member 4
Calculated molecular weight 977 aa, 112 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC104725
Gene symbol TRPC4
Gene ID (NCBI) 7223
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRPC4 antibody 21349-1-APDownload protocol
IHC protocol for TRPC4 antibody 21349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Adv

Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.

Authors - Jingjing Duan