TRPC4AP Polyclonal antibody

TRPC4AP Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 29310-1-AP

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

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Validation Data Gallery

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  WB analysis using 29310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29310-1-AP (TRPC4AP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 29310-1-AP

  IP result of anti-TRPC4AP (IP:29310-1-AP, 4ug; Detection:29310-1-AP 1:800) with HepG2 cells lysate 2040 ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29310-1-AP targets TRPC4AP in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPC4AP fusion protein Ag29860
• Full Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
• Calculated Molecular Weight: 797 aa, 91 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC013144
• Gene Symbol: TRPC4AP
• Gene ID (NCBI): 26133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.