TRPC4AP Polyclonal antibody

TRPC4AP Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 29310-1-AP

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS



Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

29310-1-AP targets TRPC4AP in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC4AP fusion protein Ag29860
Full Name transient receptor potential cation channel, subfamily C, member 4 associated protein
Calculated molecular weight 797 aa, 91 kDa
Observed molecular weight85-90 kDa
GenBank accession numberBC013144
Gene symbol TRPC4AP
Gene ID (NCBI) 26133
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Protocols

Product Specific Protocols
WB protocol for TRPC4AP antibody 29310-1-APDownload protocol
IP protocol for TRPC4AP antibody 29310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols