TRPC4AP Monoclonal antibody

TRPC4AP Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2C10B1

Cat no : 66138-1-Ig

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS



Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66138-1-Ig is TRPC4AP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen TRPC4AP fusion protein Ag18772
Full Name transient receptor potential cation channel, subfamily C, member 4 associated protein
Calculated molecular weight 797 aa, 91 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC013144
Gene symbol TRPC4AP
Gene ID (NCBI) 26133
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Protocols

Product Specific Protocols
WB protocol for TRPC4AP antibody 66138-1-IgDownload protocol
IHC protocol for TRPC4AP antibody 66138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols