TRPC5 Polyclonal antibody

TRPC5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25890-1-AP

Synonyms

hTRP 5, hTRP5, Transient receptor protein 5, TRP 5, TRP5, TRPC5



Tested Applications

Positive WB detected inHL-60 cells, MCF-7 cells
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25890-1-AP is TRPC5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC5 fusion protein Ag23171
Full Name transient receptor potential cation channel, subfamily C, member 5
Calculated molecular weight 111 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC137271
Gene symbol TRPC5
Gene ID (NCBI) 7224
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.

Protocols

Product Specific Protocols
WB protocol for TRPC5 antibody 25890-1-APDownload protocol
IHC protocol for TRPC5 antibody 25890-1-APDownload protocol
IF protocol for TRPC5 antibody 25890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

An abnormal TRPV4-related cytosolic Ca(2+) rise in response to uniaxial stretch in induced pluripotent stem cells-derived cardiomyocytes from dilated cardiomyopathy patients.

Authors - Jun Lu
mouseIF

Biochem Pharmacol

Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries.

Authors - Cai Liang
humanWB

J Mol Cell Cardiol

TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.

Authors - Zhichao Li
humanWB

Elife

Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.

Authors - Kangcheng Song