TRPC5 Polyclonal antibody

TRPC5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 25890-1-AP

Synonyms

hTRP 5, hTRP5, Transient receptor protein 5, TRP 5, TRP5, TRPC5

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Validation Data Gallery

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  WB analysis using 25890-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25890-1-AP (TRPC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25890-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25890-1-AP (TRPC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cerebellum using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 25890-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25890-1-AP (TRPC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells
• Positive IHC detected in: human brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IF: See 3 publications below

Product Information

The immunogen of 25890-1-AP is TRPC5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPC5 fusion protein Ag23171
• Full Name: transient receptor potential cation channel, subfamily C, member 5
• Calculated molecular weight: 111 kDa
• Observed molecular weight: 105 kDa
• GenBank accession number: BC137271
• Gene symbol: TRPC5
• Gene ID (NCBI): 7224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.

Publications

Species	Application	Title
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin
human	WB	Elife; Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.; Authors - Kangcheng Song
mouse	WB,IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	WB	J Mol Cell Cardiol; TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.; Authors - Zhichao Li
mouse	IF	Biochem Pharmacol; Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries.; Authors - Cai Liang
human,mouse	WB	Biochem Biophys Res Commun; Trpc5-regulated AMPKα/mTOR autophagy pathway is associated with glucose metabolism disorders in low birth weight mice under overnutrition; Authors - Fei Zhou

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