Validation Data Gallery
|Positive WB detected in||HL-60 cells, MCF-7 cells|
|Positive IHC detected in||human brain tissue, human cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25890-1-AP is TRPC5 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRPC5 fusion protein Ag23171|
|Full Name||transient receptor potential cation channel, subfamily C, member 5|
|Calculated molecular weight||111 kDa|
|Observed molecular weight||105 kDa|
|GenBank accession number||BC137271|
|Gene ID (NCBI)||7224|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.
TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.
Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.
Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.
J Mol Cell Cardiol
TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.
Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries.
Biochem Biophys Res Commun
Trpc5-regulated AMPKα/mTOR autophagy pathway is associated with glucose metabolism disorders in low birth weight mice under overnutrition