TRPC6 Recombinant monoclonal antibody

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• TRPC6 Recombinant monoclonal antibody (84517-5-RR)

TRPC6 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84517-5-RR

Clone No.251552A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6

• 

  WB analysis using 84517-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84517-5-RR (TRPC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 84517-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRPC6 antibody (84517-5-RR, Clone: 251552A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, COLO 320 cells, mouse lung tissue, human placenta tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84517-5-RR targets TRPC6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: transient receptor potential cation channel, subfamily C, member 6
• Calculated Molecular Weight: 931 aa, 106 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC093658
• Gene Symbol: TRPC6
• Gene ID (NCBI): 7225
• RRID: AB_3743577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y210
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).